In biology, techniques that can identify and localise DNA and RNA sequences are highly valued, used to detect certain genes in chromosomes, study developing organisms, characterise abnormalities, as well as diagnose diseases caused by genetic or chromosomal mutations.
If the sequence of a gene of interest is known, a complementary sequence to that gene can be synthetically designed, which will hybridize to that gene in situ. By attaching fluorescent markers to the probe, microscopy can be used to detect and quantify these probes, this is the concept behind fluorescence in situ hybridization, or FISH.
Figure 1: DNA structure, showing a chromosome and complementary dsDNA.
The FISH assay was developed in the early 1980’s and has seen widespread use ever since, due to alternate genetic analysis techniques often being more time‑consuming, and FISH gaining recognition as a genetic mapping technique related to the Human Genome Project. FISH probes come in variety of shapes and sizes, depending on the target of interest, and can be used to detect anything from single nucleotide deletions to entire chromosomes.
FISH Assay Mechanisms
Firstly, FISH probes are designed. These probes can be made from DNA, RNA, or even synthetic nucleic acids, but it must be complementary to the target of interest, both in length and sequence. Once the genetic sequence is developed, fluorophores are added, creating a fluorescent probe for a FISH assay. Additional steps can be taken to amplify the number of probes for more signal, such as using polymerase chain reaction (PCR).
In order for the probe to hybridize to the desired target DNA/RNA, both the probe and target are heated to ~90 °C, denaturing the nuclei acid and causing complementary strands to come apart. The temperature is decreased, allowing the probe to hybridize to the exposed complementary strand of the target. Washing steps remove any FISH probes that did not successfully hybridize, while the remaining probes are detected using fluorescence microscopy.
Figure 2: Examples of FISH assay images. A) BCR (green) and ABL (red) genes within chromosomes (blue) of a single cell (Bcrablmet). B+C) Her2 genes (red) and chromosome centromeres (green) within breast cancer cells (blue) (FISH Her2). D) Chromosomes within barley cells (H. vulgare).
As FISH is quite a general technique, the applications are varied and depend on both the target sequence (from a single base pair up to an entire chromosome), and the desired specificity of the FISH probe.
FISH probe specificity can be fine-tuned so that the probe will only hybridize if it is the exact complement to the target sequence, some probes will not hybridize if even one nucleotide is missing or changed (due to point deletions, copy errors or mutations), especially useful for studies in oncology and genetic syndromes, where FISH assays can potentially identify problems with sequences that can lead to disease.
Other FISH probes can be less specific, used to identify certain genes with a margin for error, or to identify whole chromosomes. The latter technique is useful for karyotyping and chromosomal studies, such as identifying diseases like trisomy 21 (Down’s syndrome), studying sperm cells, and researching specific parts of chromosomes such as the centromere and telomeres.
Due to the combination with microscopy, FISH can be used to visually identify diseased cells from a large population of cells, with images fed into detection algorithms or sent to trained personnel for detection and diagnosis. A combination of FISH with microfluidics (also termed µFISH or MA-FISH) can lead to portable diagnostic lab-on-a-chip capabilities, useful in clinical environments.
FISH serves as an experimental platform that can be modified to suit specific research purposes, over time many variations have emerged:
- mFISH: Multicolor FISH uses a range of different fluorophores to label multiple different targets with different labels, often used to label each chromosome or part of each chromosome with a different label
- Q-FISH: Quantitative FISH uses synthetic DNA mimics known as peptide nucleic acid (PNA), which only hybridize under highly specific conditions, producing more quantitative data than standard FISH assays (Poon et al. 2001).
- FRET-ISH: Förster/Fluorescence resonance energy transfer assisted in situ hybridisation, a combination that uses the sensitivity of FRET and the specificity of FISH for a powerful bio‑detection platform (Packard et al. 2012).
- smFISH: Single-molecule FISH, which provides quantitative measurements of RNA expression and the spatial localization of this expression in a spatial omics application, down to single-cell resolution (Gaspar et al. 2015).
- MERFISH: Multiplexed error-robust FISH, a multiplexed version of smFISH that assigns barcode labels to individual RNA species and uses sequential smFISH to read out these barcodes (Xia et al. 2019).
smFISH and MERFISH are also involved in high-content screening (HCS) for RNA, providing quantitative information of RNA expression and localization with multiple fluorophores across huge numbers of cells, typically with multiwell plates, high resolution imaging systems, and FISH analysis software tools and algorithms. These HCS FISH studies are useful for pharmacology and cell therapy studies, but can involve high cost RNA FISH probes (Querido et al 2017).
FISH is a technique that allows for fluorescent labelling of specific parts of the genome, from single genes to entire chromosomes, which can then be observed using microscopy. FISH found popularity during the Human Genome Project and is now widely-used in medical and research settings for detection, localisation, diagnosis and study of how genetic information can change during development. FISH now exists in many variations and is a powerful platform linking fluorescence microscopy with genomics.
Gaspar, I., & Ephrussi, A. (2015). Strength in numbers: quantitative single-molecule RNA detection assays. Wiley interdisciplinary reviews. Developmental biology, 4(2), 135–150. https://doi.org/10.1002/wdev.170
Packard, M. M., Shusteff, M., & Alocilja, E. C. (2012). Microfluidic-Based Amplification-Free Bacterial DNA Detection by Dielectrophoretic Concentration and Fluorescent Resonance Energy Transfer Assisted in Situ Hybridization (FRET-ISH). Biosensors, 2(4), 405–416. https://doi.org/10.3390/bios2040405
Poon, S., & Lansdorp, P. M. (2001). Quantitative fluorescence in situ hybridization (Q-FISH). Current protocols in cell biology, Chapter 18, 18.4.1–18.4.21. https://doi.org/10.1002/0471143030.cb1804s12
Querido, E; Dekakra-Bellili, L; Chartrand, P (2017). RNA fluorescence in situ hybridization for high-content screening. Methods, S1046202316304212 – doi:10.1016/j.ymeth.2017.07.005
Xia, C., Babcock, H.P., Moffitt, J.R., & Zhuang X. (2019) Multiplexed detection of RNA using MERFISH and branched DNA amplification. Sci Rep 9, 7721. https://doi.org/10.1038/s41598-019-43943-8